Fig. 2

Characterization of the immortalized zebra finch cell line. A Alcian blue staining of primary zebra finch embryonic fibroblasts (ZEFs) and the immortalized cell line. Scale bar, 100 μm. B Gene expression profiling of the primary ZEFs, the immortalized zebra finch cell line, and primary chondrocyte-like cells (CLCs). DW, distilled water. C Quantitative RT-PCR analyses of the primary and immortalized cells; *p < 0.05, ***p < 0.0005, ****p < 0.0001