Fig. 6

Distribution of Sulcia, YLS and Hodgkinia in the freshly laid eggs, nymphs and adults of three representative cicada species. A to C Both Sulcia and the coresident symbiont (i.e., YLS or Hodgkinia) were intermixed within the “symbiont ball” in the freshly laid eggs of E. repanda, C. atrata and H. maculaticollis. D Sulcia and Hodgkinia were harbored in the bacteriomes of the newborn first-instar nymphs of E. repanda. E to F YLS was initially harbored in the bacteriome sheath of bacteriomes in the newborn first-instar nymphs of C. atrata and H. maculaticollis. G to L Sulcia was harbored in the bacteriomes of adults of three cicada species, and Hodgkinia was present in the bacteriomes of E. repanda adults. In regard to the Hodgkinia-free cicadas, YLS is harbored in the fat bodies of C. atrata adults and in both the bacteriomes and fat bodies of H. maculaticollis adults. Abbreviations: H, Hodgkinia; S, Sulcia; Y, yeast-like fungal symbiont; SB, symbiont ball