Table 1 Primers used in this study for AmelOBP4 double-stranded RNA synthesis and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analyses
gene | Application of primers | Primer sequence (5’–3’) |
|---|---|---|
AmelOBP4 | Primer for qPCR | F: GAGTCTGGAACTCGAGAACTAACACC R: CAACCATGCATTCGTCTTCGTCTG |
β-actin | F: TGCCAACACTGTCCTTTCTG R: AGAATTGACCCACCAATCCA | |
dsAmelOBP4 | Primer RNA interference | F: AGCAATTCTATGCTCGCAAAA R: CATCCTCCGTAAAGTCGTCG |
T7F:taatacgactcactatagggAGCAATTCTATGCTCGCAAAA T7R: taatacgactcactatagggCATCCTCCGTAAAGTCGTCG | ||
dsGFP | F: taatacgactcactatagggGTGGAGAGGGTGAAGG R: taatacgactcactatagggGGGCAGATTGTGTGGAC |