Fig. 2

Assembly quality assessment with blobtoolkit. A) Blobplot of FCS filtered contigs: Each circle represents a sequence of the assembly. Size and color of the circle correspond to the size and taxonomic assignment of the respective sequence. Note that the cluster of contamination on the bottom left contains sequences assigned to Ciliophora and no-hits (amongst others), which were not filtered out by FCS. B) Snailplot of chromosome scale scaffolds: Graphical representation of various contiguity statistics of a genome assembly. Main plot (center): Clock wise the absolute and relative length of the assembly is displayed. The outer light and dark blue ring show GC content at a respective position of the assembly. Dark grey columns in the middle show the number of the sequence and its length with height and angle, respectively. Major contiguity statistics as longest sequence, N50 and N90 are highlighted in red and orange tones. Additionally, on the top right a graphical representation of BUSCO results is shown (Figure reproduced from Männer et al. 2024)